• Why do the GlutenTox gluten test kits work?
• What is difference between the G12 antibody used in GlutenTox and other antibodies?
• What is the difference between GlutenTox Pro and GlutenTox Sticks?
• What is the GlutenTox Reader?
• Is there a GlutenTox ELISA test?
• Is there a smaller version of GlutenTox? I need fewer than 25 tests.
• I own a business outside of the US. Do you ship to___?
• Will you analyze my samples for gluten if I mail them to you?
• How can I test surfaces for gluten contamination?
• How should I dispose of the kit after use?
• At what temperature should I store the kit?
• What is the expiration date of the kit?
• Who makes/distributes GlutenTox?
• I got a test result that surprises me. What do I do?
• What happens if the toxic fragment of the gluten molecule is cleaved during the hydrolyzation process?
• What kind of safety qualifications does GlutenTox have?
• How do I pronounce “immunochromatographic”?
• Where can I read more about the science behind GlutenTox and the G12 antibody?
What is GlutenTox?
GlutenTox is a series of user-friendly lateral flow tests that detect gluten contamination from wheat, barley, rye and even oats in foods and liquids, and on surfaces. GlutenTox Home, Pro, and Sticks all use the G12 antibody, and are each designed to accommodate the testing needs of differently sized businesses. ^
Why do the GlutenTox gluten test kits work?
The G12 antibody, located inside the test strip, is specially designed to seek out the 33-mer peptide of the alpha-gliadin molecule. Studies show that this peptide is the primary cause of the auto-immune response in celiac patients. Accordingly, a food will test positive with GlutenTox if it contains these toxic peptides.
G12 is able to recognize gluten in wheat, barley, rye and oat (for more on oat toxicity, see below). It is also uniquely well-suited to detecting gluten in hydrolyzed, fermented, or otherwise processed foods. ^
What is difference between the G12 antibody used in GlutenTox and other antibodies?
The G12 antibody specifically recognizes the most toxic fragment of the gliadin present in gluten, called 33-mer. Because gliadin is often hydrolyzed in foods and drinks, less-specific antibodies may underestimate the amount of net toxic fractions. In addition, the G12 antibody has never shown any cross-reactivity with soy or other gluten-free foods. In all cases, the G12 antibody will always be able to detect if a sample is toxic for celiac patients because it specifically detects that toxic fraction; it is therefore much more reliable than classic antibodies for hydrolyzed food such as cookies, beer, baby food, etc. ^
What is the difference between GlutenTox Pro and GlutenTox Sticks?
There are several differences. GlutenTox Pro is right for you if you:
- Do not have access to standard lab equipment; GlutenTox Pro does not require any special equipment and everything is included in the box
- Require results in the shortest time possible (less than 20 minutes)
GlutenTox Sticks are right for you if you:
- Are comfortable using basic lab equipment like a micropipette, test tubes, wheel agitator, etc.
- Perform a high volume of tests
- Are interested in using the GlutenTox Reader to provide a precise quantification of the gluten content in your samples
- Are testing items containing chocolate or with especially complex matrices
- Prefer to test to 3ppm instead of 5ppm
We’re happy to talk with you to determine which kit is the best fit for your facility. ^
What is the GlutenTox Reader?
GlutenTox Reader is a portable device that provides a precise analysis of PPM in a sample. It works in conjunction with GlutenTox Sticks; a processed test strip is simply placed in the Reader and within seconds a quantified result is available digitally. ^
Is there a GlutenTox ELISA test?
There are ELISA tests that contain the G12 antibody, however Emport, LLC does not carry them. If you are interested in running ELISA tests in your lab, we will happily connect you with the proper contacts. ^
Is there a smaller version of GlutenTox? I need fewer than 25 tests.
Many clients prefer to use GlutenTox Home, which is available for purchase online at our shop. GlutenTox Home can be ordered in increments of 2, 5, or 10 tests and generally ships out within 24 hours of the order being placed online. ^
I own a business outside of the US. Do you ship to___?
Emport, LLC primarily ships to addresses within North America. However, GlutenTox is distributed around the world; if we are unable to ship to your location we will happily connect you with the appropriate distributor or with Biomedal directly. ^
Will you analyze my samples for gluten if I mail them to you?
Sorry, but Emport, LLC is not equipped to perform in-house analysis of samples, nor can we issue certifications about the gluten content of your items. We are, however, able to offer analysis performed in Biomedal’s ISO-certified laboratory in Spain. Kindly contact us for more information. ^
How do I test a solid or liquid sample for gluten?
How can I test surfaces for gluten contamination?
How should I dispose of the kit after use?
All components of the kit are non-toxic and therefore fully disposable in ordinary trash. They can be recycled where appropriate according to the material. ^
At what temperature should I store the kit?
The kit can be stored at 35°F – 85°F. ^
What is the expiration date of the kit?
Expiration dates are clearly printed on each kit, but are generally at least a year from the ship date. You will know if you are using a test that has expired because the blue control line will not appear. ^
Who makes/distributes GlutenTox?
All GlutenTox kits are made by Biomedal Diagnostics, in sunny Seville, Spain. Biomedal is a fully accredited facility, holding ISO certifications for the production of gluten test kits as well as on-site gluten analysis.
Emport, LLC distributes GlutenTox in North America. Emport has one simple goal: more safe food for more happy people. The company is based in cloudy Pittsburgh, PA. ^
How sensitive are the GlutenTox tests?
You can choose the sensitivity of each test you perform. GlutenTox Pro has a minimum detection limit of 5ppm, and GlutenTox Sticks can be set as low as 3ppm. The tests can also be set for higher thresholds, allowing for semiquantitative analysis: for example, if you have a positive result at 5ppm you can run a second test of the same sample to a higher threshold like 20ppm. If the second test is negative, you will know that your sample contains between 5 and 20 ppm of gluten.
According to the Codex Alimentarius Commission, food can be considered “gluten-free” if the amount of gluten does not exceed 20 parts per million (ppm), and is considered as “very low in gluten content” if the amount of gluten does not exceed 100 ppm. ^
Does the test recognize oat?
Yes. The test can detect both cross-contamination in oats, and also those strains of oat that contain gluten. A positive signal will appear if the oat being tested is potentially harmful for celiac disease patients. If you’d like to read more about gluten in oats, we recommend this study on gluten in oats. You can also visit Celiac.com for an analysis of the study. ^
Why is GlutenTox Pro not recommended for chocolate?
Gluten from chocolate or cocoa is very hard to extract; for this reason the level of gluten can be underestimated. However, if you get a positive result with chocolate, it definitely does contain gluten and should not be consumed by anyone on a gluten-free diet.
There is an additional buffer available for use with GlutenTox Sticks, which addresses the phenol structure of cocoa. Contact us for more information. ^
Can I test the oil in my deep fryer with GlutenTox Pro?
Oil is inherently gluten-free, and the primary risk of cross-contamination comes from the gluten-containing crumbs that may be floating in your oil and could potentially adhere to otherwise gluten-free food. The recommended process for verifying that a deep fryer or fryolater is safe for use is to filter the oil and collect the crumbs and other residue that you find. Pat and/or rinse the residue to remove as much excess oil as possible, and then test the residue using GlutenTox standard procedures for crumbs or powders. If the residue produces a negative test, you can consider the oil safe for use with gluten-free items.^
Are there other items GlutenTox is not recommended for?
- Maggi – Seasoning – Arôme (liquid sauce): This seasoning sauce contains gluten as described by the manufacturer in its ingredients list. Note that its manufacturing process and other active ingredients are not suitable for GlutenTox Home. Consider this food as containing gluten.
- Soy Sauce: Naturally brewed soy sauce undergoes fermentation process that often hydrolyzes/breaks down gluten to level close or below 20 ppm; a negative result on a sample containing soy sauce indicates that the toxic peptides have been broken down.
- Highly processed and complex matrices, as well as those with polyphenols and/or antioxidants: Although GlutenTox tests have been successfully tested against a wide variety of highly-processed non-food items, there may be some cases in which a more thorough extraction process is required to identify traces of gluten. If you are planning to test a vitamin, supplement, medication, or products with polyphenols (chocolate, tannins, etc.) that you feel may be highly processed, please contact us with more information about the specific item. In all cases, a positive test result always indicates the presence of gluten: there are no false positives.
- Supplements or personal care products that are very high in silica should not be tested using GlutenTox, and often require special analysis in a laboratory setting. ^
After 10 minutes, the test showed a negative result (only the blue control line appeared). But when I checked the test strip the next day, I noticed a faint pink line. What’s going on?
The test results should be read at 10 minutes (not before and not after). Any faint lines that appear after this time limit are not valid and do not indicate the presence of gluten in the sample. Conversely, positive results may fade in intensity after 10 minutes. If the test shows a pink line and a blue line at 10 minutes, it’s positive. If the test shows only a blue line at 10 minutes, it’s negative.
In the case of a sample that does contain high amounts of gluten, you may see the pink line appear before 10 minutes have elapsed; assuming the blue control line is also present, it is safe to declare the test positive. However, a test cannot be declared negative before 10 minutes have elapsed. ^
I got a test result that surprises me. What do I do?
If your results are unexpected, please email us with more information about your test. We’ll work with you until your questions are resolved. ^
What happens if the toxic fragment of the gluten molecule is cleaved during the hydrolyzation process?
Being cleaved, the fragment will not be detected by the G12 antibody — BUT it will also lose its toxicity. This sample is therefore suited for celiac patients (Morón et al. 2008). ^
What kind of safety qualifications does GlutenTox have?
Biomedal Diagnostics has numerous positive evaluations for its gluten test kits and in-house gluten testing: AESAN (The Spanish Agency for Food Safety and Nutrition) has positively evaluated the GlutenTox Lateral Flow Device as an accurate method for gluten detection in any types of food. AESAN is an organization attached to the Spanish Ministry of Heath and Social Politics, whose role is to ensure the highest standards for safety and promote citizens health. The test kits have also passed the FAPAS proficiency tests, an international standard based in the UK. Additionally Biomedal holds both ISO 9001 and ISO 17025 certifications. ^
How do I pronounce “immunochromatographic”?
We think it’s a pretty neat word too. The IPA (International Phonetic Alphabet) rendition would be: